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Fundamentals of Laboratory Biotechnology

6
  • Pipetting: Precision Liquid Handling
  • Aseptic Technique: Preventing Contamination
  • Sterilization Methods: Ensuring Aseptic Environments
  • Using a Centrifuge: Separating Biomolecules
  • Preparing Buffers: The Chemical Backbone of Experiments
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Genetic Engineering & Synthetic Biology

2
  • Gene Synthesis: Custom DNA Construction
  • CRISPR-Cas9: Precision Genome Editing
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Protein-Level Analyses

4
  • Mass Spectrometry (Proteomics): Protein Identification and Quantification
  • 2D Gel Electrophoresis: High-Resolution Protein Separation
  • ELISA (Enzyme-Linked Immunosorbent Assay): Antigen/Antibody Detection
  • Western Blotting: Protein Detection and Quantification
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Techniques in Molecular Genetics

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  • RNA-seq: Comprehensive Transcriptome Analysis
  • Microarrays: High-Throughput Gene Expression Profiling
  • Reverse Transcription PCR (RT-PCR): RNA to DNA Analysis
  • Quantitative PCR (qPCR): Real-time Gene Quantification
  • Cloning: Recombinant DNA Construction
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